[HTML][HTML] Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing

MC Lucas, LP Pryszcz, R Medina, I Milenkovic… - Nature …, 2023 - nature.com
tRNA populations that provides quantitative estimates of both … tRNA constructs (IVT Drosophila
melanogaster mitochondrial … mapped IVT tRNAs, it failed to map a single biological tRNA

Non-redundant tRNA reference sequences for deep sequencing analysis of tRNA abundance and epitranscriptomic RNA modifications

F Pichot, V Marchand, M Helm, Y Motorin - Genes, 2021 - mdpi.com
… For better efficiency, the tRNA population may be fractioned … and thus very ambiguous
mapping of tRNA reads, we foresee … Panels (A,B) show analysis of Drosophila melanogaster and …

tRNA 2′-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila

MT Angelova, DG Dimitrova, B Da Silva… - Nucleic Acids …, 2020 - academic.oup.com
… mass spectrometry and RiboMethSeq mapping revealed that … to yeast and humans, Drosophila
melanogaster has evolved two … For northern blotting analysis of tRNA, 10 μg of total RNA …

[HTML][HTML] Optimization of ribosome footprinting conditions for Ribo-Seq in human and Drosophila melanogaster tissue culture cells

K Douka, M Agapiou, I Birds, JL Aspden - Frontiers in Molecular …, 2022 - frontiersin.org
analysis, reads were globally mapped to 5′ and 3′ UTRs and coding regions (CDS) and
the mean number of reads that is mapped … originating from rRNA and tRNA were aligned and …

Rapid evolution of piRNA clusters in the Drosophila melanogaster ovary

S Srivastav, C Feschotte, AG Clark - bioRxiv, 2023 - biorxiv.org
… In the present study, we found that there is extensive … We present normalized coverage of
uniquely mapping piRNAs to the … melanogaster, we used population genomics 481 methods to …

Transcriptome-wide analysis of pseudouridylation in Drosophila melanogaster

W Song, R Podicheti, DB Rusch, WD Tracey Jr - G3, 2023 - academic.oup.com
… Enzymatic Psi modification occurs on rRNA, snRNA, snoRNA, tRNA, and non-… Drosophila
melanogaster. Here, we optimized Psi-seq … The RNA samples we used for the mapping of …

The PPR domain of mitochondrial RNA polymerase is an exoribonuclease required for mtDNA replication in Drosophila melanogaster

Y Liu, Z Chen, ZH Wang, KM Delaney, J Tang… - Nature Cell …, 2022 - nature.com
… We thus performed hydrolysis-based tRNA sequencing and found a … The genotypes of all fly
lines used in this study are listed in … profile was calculated based on uniquely mapped reads. …

[HTML][HTML] Off-target piRNA gene silencing in Drosophila melanogaster rescued by a transposable element insertion

DE Miller, AP Dorador, K Van Vaerenberghe, A Li… - PLoS …, 2023 - journals.plos.org
… 30 nt and miRNA/tRNA/rRNA depleted using … analysis, 23 to 30 nt small RNA reads were
mapped with bwa to release 6.33 of the Drosophila melanogaster reference, counts analyzed

ELAC2/RNaseZ-linked cardiac hypertrophy in Drosophila melanogaster

E Migunova, J Theophilopoulos… - Disease Models & …, 2021 - journals.biologists.com
… This study provides first experimental evidence for the pathogenicity of … The most prevalent
form is HCM, with one in 500 people … ELAC2/RNaseZ endoribonuclease is essential for tRNA

[HTML][HTML] Evidence of the Physical Interaction between Rpl22 and the Transposable Element Doc5, a Heterochromatic Transposon of Drosophila melanogaster

MF Berloco, CF Minervini, R Moschetti, A Palazzo… - Genes, 2021 - mdpi.com
… In silico analyses reveal that Doc5 maps exclusively in the constitutive heterochromatin of
… that RP complexes are involved in tRNA biogenesis and possibly centromere functions [57]. …